Analysis of Omeprazole and Lansoprazole in Capsules by Capillary Zone Electrophoresis

 

A simple capillary zone electrophoresis method is discussed for the simultaneous analysis of omeprazole and lansoprazole.
A simple capillary zone electrophoresis method (CZE) was established for simultaneous analysis of omeprazole (OM) and lansoprazole (LA). Untreated fused-silica capillary was operated using a phosphate buffer (50 mM, pH 9.0) under 20 kV and detection at 200 nm. Baseline separation was attained within 6 min. In method validation, calibration curves were linear over a concentration range of 5 to 100 µM, with correlation coefficients 0.9990. Relative standard deviation (RSD) and relative error (RE) were all less than 5% for the intra-day and inter-day analysis, and all recoveries were greater than 95%. The limits of detection for both omeprazole and lansoprazole were 2.0 µM (S/N = 3, hydrodynamic injection 5 s). This method was applied to determine the quality of commercial capsules. Assay results fell within 94–106%.

Author(s): 

Yi-Hui Lin,a and Shou-Mei Wu,a,b,c aGraduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, bFaculty of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, cFaculty of Fragrance and Cosmetics, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

Journal: 

LCGC Europe, LCGC Europe, Mar 1, 2005

Copyright: 

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