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Friday, April 20, 2012

Reversed-Phase Liquid Chromatographic Method for Simultaneous Estimation of Artemether and Lumefantrine in Combined Dosage form By Ritesh lathiya, Laukik patel

 Reversed-Phase Liquid Chromatographic Method for Simultaneous Estimation of Artemether and Lumefantrine in Combined Dosageform.
Ritesh lathiya,
Laukik patel
.
Department of Pharmaceutical analysis, pacific University, Udaipur,Rajasthan, India.
Corres.author:riteshprajapati777@gmail.com,
Tel.: +919724969295
Abstract:

Abstract: A simple, rapid, precise and accurate reversed phase high performance liquid chromatographic method has been developed for the simultaneous determination of Artemether in combination with Lumefantrine. This method uses a Hypersil ODS C18(250mm×4.6mm×5μ particle Size) analytical column, a mobile phase of methanol: 0.05 % trifluroacetic acid with triethylamine buffer pH 2.8 adjusted with orthophosphoric acid in ratio (80:20 v/v). The instrumental settings are a flow rate of 1.5 ml/min and PDA detector wavelength at 210 nm. The retention times for Artemether and Lumefantrine are 6.15 min and 11.31min, respectively. The method is validated and shown to be linear. The linearity range for Artemether and Lumefantrine are 20-120 & 120-720 μg/ml respectively. The Percentage recovery for Artemether and Lumefantrine are ranged between 99.50–101.16 and 99.78–101.21 respectively. The correlation coefficients of Artemether and Lumefantrine are 0.999, and 0.999, respectively. The relative standard deviation for six replicates is always less than 2%. The Statistical analysis proves that the method is suitable for analysis of Artemether and Lumefantrine as a bulk drug and in pharmaceutical formulation without any interference from the excipients.
Key words - Artemether, Lumefantrine, Validation and RP-HPLC.


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