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Tuesday, August 9, 2011

Development and validation of a HPTLC method for the simultaneous estimation of telmisartan and hydrochlorothiazide in tablet
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the estimation of telmisartan and hydrochlorothiazide simultaneously in combined dosage forms. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of chloroform: methanol: toluene (2:5:5 v/v/v). The detection of spots was carried out at 272 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 250 to 500 ng/spot for telmisartan and 200 to 700 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for the telmisartan were found to be 75 and 190 ng/spot, respectively, and for hydrochlorothiazide 55 and 150 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.
NJ Shah1, BN Suhagia2, RR Shah1, PB Shah3
Indian Journal of Pharmaceutical Sciences,Year : 2007 | Volume : 69 | Issue : 2 | Page : 202-205


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